Izifo Ezibangelwa Amagciwane (Chemiluminecense Immunoassay)

Izingxenye ezihlukahlukene zezinto ezingezwani nemvelo zizoshukumisa umzimba ukuthi ukhiqize amasosha omzimba athile e-IgE;njengezibungu zothuli lwendlu, izingxenye ezingaphezu kuka-30 ze-allergenic zihlonziwe futhi zibonakaliswe, kufaka phakathi i-tropomyosin yezibungu zothuli lwendlu, ama-Enzyme ahlukahlukene, amaprotheni okulawula, amaprotheni esakhiwo, njll;njengobisi, izingxenye eziyinhloko ze-allergenic yi-casein, i-α-lactalbumin, i-β-lactoglobulin, njll. Lezi zingxenye ngazinye ziyizingxenye ze-allergen.

Izindlela zokuxilonga zendabuko zisebenzisa i-allergen ekhishwayo engahluziwe, ekala "isamba" se-IgE esiboshelwe kuzo zonke izingxenye zamaprotheni kuyo yonke i-allergen;ukuxilongwa kwengxenye-analytical kusebenzisa i-allergen ehlanzekile yemvelo noma izingxenye ze-allergenic recombinant ukuhlonza i-allergenic Specific IgE, okwenza ukutholakala kwezifo eziguliswayo kucace kakhudlwana.

Ukusebenza

Izinto ezingezwani nezakhi zihlanzwa nge-affinity chromatography kanye/noma i-HPLC.Ubunikazi bamaprotheni kanye nokwakheka kwe-amino acid kuqinisekiswa i-mass spectrometry kanye nokuhlaziywa kwe-amino acid.Ukusebenza kabusha kwamasosha omzimba kuqinisekiswa ngokubopha kwe-monoclonal antibody.Izifo ezithathelwanayo ezihlanziwe nazo ziyaqinisekiswa ekubopheni ama-antibody e-IgE.

Izicelo:

Izifundo ze-in vitro zomsebenzi we-T-cell kanye nokwethulwa kwe-antigen

Amamodeli ezilwane ezimpendulo ze-IgE nesifuba somoya

I-Innate Immunity

Ukutholwa kwe-antibody, ukuhlolwa kokukhishwa kwe-histamine

Izifundo zesakhiwo kanye nokuhlolwa kwezinto eziphilayo